Workshop II

Facilitator :
Assoc. Prof. Dr. Teoh Teow Chong (University of Malaya)
Dr. Mohammed Rizman Idid (University of Malaya)

Workshop II
Transcriptomic Analysis of Polar Fungi: Applications of NGS and Methods of RNA-Seq Analytics for Climate Change Studies

25 October 2021

Introduction

This 1 Day Online workshop on Next Generation Sequencing (NGS) and RNA Sequencing Data Analysis will focus on de novo and reference genome analysis pipelines. For both the pipelines, we will demonstrate how to analyse step by step the raw data, conduct data filtering, check for Quality Control (QC), assemble the transcripts, annotate the identified unigene and to analyse differential gene expression.

The online demonstration will use command lines via a virtual Linux that runs on Windows apps. Participants will be able to view the demonstration on-screen and provided with a pdf manual containing basic command lines used in the workshop.

Introductory lectures will include the experimental design of transcriptomics in polar fungi and climate change studies, and the application of Next Generation Sequencing (NGS) on fungi. This workshop is designed for bioinformaticians, biologists, biomedical researchers and postgraduate students.

Objectives

Learn to design a transcriptomic experiment on climate change.
Learn the methods of de novo and reference genome analysis.
Provide key points and tips for getting the best results.
Interpretation of the results

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Workshop I

Time (MYT, UTC+8)	Presentation / Demonstration (facilitator)
09:00 - 09:40 am	Transcriptomic Insights on Polar Fungi Response to Climate Change Dr. Mohammed Rizman Idid - (30mins) + Q&A (10mins)
09:40 - 10:20 am	*The Effect of Warming on the Transcriptomic Activity of Pseudogymnoascus* sp. from the Arctic** Assoc. Prof. Dr. Teoh Teow Chong - (30 mins + 10 mins Q&A)
10:30 am - 12:30 noon	Workshop II - PART 1: Methods in RNA-Seq analytics: reference genome and de novo approaches. A case study on heat stress responses in Pseudogymnoascus sp. -(2 hrs)
2.00 - 5.00 pm	Workshop II - PART 2: Methods in RNA-Seq analytics: reference genome and de novo approaches. A case study on heat stress responses in Pseudogymnoascus sp. -(3 hrs)